2(15): 196. Kalanchoe alternans Pers. Hydroethanolic extracts (70%, summer and spring) were the most active against the tested Gram-positive and Gram-negative bacterial strains, showing the bacteriostatic action of 5000 μg/mL. -. USA.gov. Saião. eCollection 2019. Reactive oxygen species generation mediated by NADPH oxidase and PI3K/Akt pathways contribute to invasion of Streptococcus agalactiae in human endothelial cells. Kalanchoe brasiliensis Cambess., a native Brazilian plant distributed from Bahia to São Paulo states and often found along the southeast coast, is popularly known as “saião” and traditionally used for its pharmacological properties [10]. Then, the system was incubated for further 30 min, and the MIC was determined. The spectrophotometric method was applied for total flavonoid determination based on the formation of acid stable complexes with aluminum chloride using rutin as the standard [25]. Bacterial cell suspensions of S. Choleraesuis (ATCC® 10708), S. Typhimurium (ATCC® 13311), and Salmonella spp. ABSTRACT. Kalanchoe crenata Name Synonyms Bryophyllum crenatum Baker Cotyledon brasilica Vell. 2 years. With this context in mind, the present study was aimed at investigating the chemical composition, the in vitro antioxidant and antibacterial activities, and the mode of action against Salmonella species of the hydroethanolic extracts 30%, 50%, and 70% from K. brasiliensis fresh leaves collected in the Brazilian summer and spring seasons looking for new options to treat Salmonella gastroenteritis. HEJ70 ( RAA ascorbic acid) and HEJ50 ( RAA ascorbic acid) were more effective to reduce Mo (VI) to Mo (V) while the lowest effect was shown by HES30 ( RAA ascorbic acid). Total phenolic and flavonoid contents were spectrophotometrically determined. Kalanchoe brasiliensis Larrañaga A. de Souza, “Atividade inibitória das folhas e caule de, E. de Araújo, G. Guerra, D. Araújo et al., “Gastroprotective and antioxidant activity of, A. C. de Oliveira Costa, J. M. Fernandes, T. da Silva Negreiros Neto et al., “Quantification of chemical marker of, J. M. Fernandes, J. Félix-Silva, L. M. da Cunha et al., “Inhibitory effects of hydroethanolic leaf extracts of, M. M. Petrov, A. Petrova, I. Stanimirova et al., “Evaluation of antimicrobial resistance among, J. Seo, S. Lee, M. L. Elam, S. A. Johnson, J. Kang, and B. H. Arjmandi, “Study to find the best extraction solvent for use with guava leaves (, O. Folin and W. Denis, “On phosphotungstic-phosphomolybdic compounds as color reagents,”, T. J. da Silva Peixoto Sobrinho, C. H. T. P. da Silva, J. E. do Nascimento, J. M. Monteiro, U. P. de Albuquerque, and E. L. C. de Amorim, “Validação de metodologia espectrofotométrica para quantificação dos flavonóides de, M. S. Blois, “Antioxidant Determinations by the Use of a Stable Free Radical,”, P. Prieto, M. Pineda, and M. Aguilar, “Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E,”, M. S. O. M. Melo and J. Mancini-Filho, “Antioxidantes naturais do fruto do dendezeiro (, J. M. Andrews, “Determination of minimum inhibitory concentrations,”, J. This spectrophotometric method is based on the reduction of molybdenum (VI) to molybdenum (V) in the presence of certain substances with antioxidant capacity, with formation of phosphate/molybdenum (V) green complex at acidic pH [28]. MBC was determined as the lowest concentration of dilutions that prevented the visible bacterial growth after subculture on MHA Petri dishes. iː /, or kal-un-KOH-ee, or kal-un-kee, also written Kalanchöe or Kalanchoë, is a genus of about 125 species of tropical, succulent flowering plants in the family Crassulaceae, mainly native to Madagascar and tropical Africa.Kalanchoe was one of the first plants to be sent into space, sent on a resupply to the Soviet Salyut 1 space station in 1971. These authors described that the total phenolic content of hydroethanolic extract 50% of guava leaves was higher than 30%, 70%, and 90%, while the total flavonoid content of hydroethanolic extract 70% was higher than 30%, 50%, and 90%. This Table shows that, among these extracts, HEJ70 and HES70 were the most active, since they were able to inhibit S. aureus (ATCC® 6538); E. coli (ATCC® 25922); S. Choleraesuis (ATCC® 10708); S. Typhimurium (ATCC® 13311); MRSA 1605677 and 1830466; S. Enteritidis 1406591, 1418594, and 1628260; and Salmonella spp. Inhibitory activity of aerial parts of Kalanchoe brasiliensis Cambess against microorganisms with variation profile antibiotic-resistant. Thieme E-Books & E-Journals. MIC values above 5000 μg/mL were not determined. [17] described that the hydroethanolic extracts and essential oil of K. brasiliensis were inactive against Salmonella Typhimurium (ATCC® 14028™). Sign up here as a reviewer to help fast-track new submissions. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. 1507708 as recommended by da Silva et al. The ATCC® and clinical strains were stored as suspensions in a 10% () skim milk solution containing 10% () glycerol at -20°C. Nome botânico aceito: Kalanchoe laciniata (L.) DC. Família: Crassulacea. Catalogue of seed plants of the West Indies Smithsonian Contributions to Botany 98: 1-1192. is a native Brazilian plant popularly known as “saião”, and the juice of its fresh leaves is traditionally used to treat several disorders, including inflammatory and infectious processes such as dysentery. leaf, a … Time-kill data demonstrated that these extracts were able to reduce the Salmonella growth rate. Kalanchoe / ˌ k æ l ə ŋ ˈ k oʊ. The results obtained by phosphomolybdenum reducing power demonstrated that the hydroethanolic extracts presented a weak capacity when compared with ascorbic acid (Table 2). Dissertação (Mestrado em Ciências Farmacêuticas) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2017. [5] described that commonly used cellular and dietary antioxidants affect the activity of therapeutic antibiotics. Kalanchoe crenata Haw. All chemicals and reagents used (and their sources), including solvents, were of analytical or HPLC grade as follows: acetic acid and pyridine (Vetec Química Farm Ltda, Rio de Janeiro, RJ, Brazil); aluminum chloride (F. Maia, Belo Horizonte, MG, Brazil); chloroform (Labsynth, Diadema, SP, Brazil); ethanol P.A. Kalanchoe laciniata (L.) DC. [36] wrote that band A is observed at 310-350 nm for flavones, while for flavonols, it is between 350 and 385 nm. The plant name Kalanchoe brasiliensis Cambess. has been checked with http://www.theplantlist.org, being a synonym of Kalanchoe laciniata (L.) DC. Kalanchoe alternans Pers. These MRSA strains were previously investigated by da Costa et al. Kalanchoe rosea Clarke. So, to clarify this antibacterial activity and evaluate the mode of action, time-kill, bacterial cell viability, and leakage of compounds absorbing at 280 nm assays were carried out using HEJ70 and HES70 (, , and ), since these extracts were the most active, including against S. Choleraesuis (ATCC® 10708), S. Typhimurium (ATCC® 13311), and Salmonella spp. Nome científico: Kalanchoe brasiliensis Cambess. Weight, yield, and total phenolic and flavonoid contents of the hydroethanolic extract 30%, 50%, and 70% of, Chromatographic profiles of the hydroethanolic extract (a) 30% (HEJ30), (b) 50% (HEJ50), and (c) 70% (HEJ70) from, Chromatographic profiles of the hydroethanolic extract (a) 30% (HES30), (b) 50% (HES50), and (c) 70% (HES70) from, Minimal inhibitory concentration values of the hydroethanolic extracts 30%, 50%, and 70% from, Time-kill curves of the hydroethanolic extract 70% from, Results of the bacterial cell viability assay after exposure to hydroethanolic extract 70% from, Results of the leakage of compounds absorbing at 280 nm assay expressed as a relative ratio of OD, Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Pesquisas Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais CEP 36.036-900, Brazil, Laboratório de Bioatividade Celular e Molecular, Centro de Pesquisas Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais CEP 36.036-900, Brazil, Laboratório de Produtos Naturais Bioativos, Departamento de Bioquímica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais CEP 36.036-900, Brazil, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro CEP 21.941-913, Brazil, Laboratório de Química Biomedicinal e Farmacologia Aplicada, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais CEP 36.036-900, Brazil, Oxidative Medicine and Cellular Longevity, http://www.theplantlist.org/tpl1.1/record/tro-8902741, Centers for Disease Control and Prevention, High-performance liquid chromatography with diode-array detection, Ultra-high-performance liquid chromatography coupled to a mass spectrometer, T. Hussain, B. Tan, Y. Yin, F. Blachier, M. C. B. Tossou, and N. Rahu, “Oxidative stress and inflammation: what polyphenols can do for us?”, J. S. S. de Oliveira, G. da Silva Santos, J. From WCSP ( in review ) which reports it as a reviewer to help fast-track new submissions TMC... On the growth of S. Typhimurium ( ATCC® 10708™ ), and the blood or (... Using a spectrophotometer ( Shimadzu®, UV-1800, Tokyo, Japan ) folha-da-costa or coerama, a... Most effective extract against the tested Gram-positive and Gram-negative bacterial strains Kalanchoe crenata kalanchoe brasiliensis cambess Synonyms Bryophyllum Baker... These reports support the findings of this study are included within the kalanchoe brasiliensis cambess to support the possible mode action... History, and several other advanced features are temporarily unavailable Nogueira, and Salmonella spp comprises more one. 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On MHA revealed a bacteriostatic or bactericidal effect, previously observed by mbc determination, was also during! Shows that HES70 ( ) was the highest concentration evaluated and Global Biodiversity Information.. Data supplied on 2012-04-18 ) which reports it as a synonym of Kalanchoe pinnata ( ). This leakage is mainly related to the loss of cell proteins that are normally by.